| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 178993 | Electrochemistry Communications | 2014 | 4 Pages |
•Preparation of a yeast-modified electrode•Application of a yeast-modified electrode for the quantitative determination of NADP+•Reversible voltammetry of NADP+•Surface mass changes during the cyclic voltammetry of NADP+
The dominant voltammetric response of a yeast suspension in neutral or slightly alkaline media can be assigned to the redox transformations of nicotinamide adenine dinucleotide (NAD+/NADH) and nicotinamide adenine dinucleotide phosphate (NADP+/NADPH). By immobilization of yeast on platinum, a stable electrode can be prepared which shows an electrocatalytic activity towards the reduction of NADP+ and the reoxidation of the product formed. Reversible cyclic voltammetric responses were obtained. The peak currents depend practically linearly on the NADP-Na2 concentration and on the square root of the scan rate. The surface mass changes accompanying the redox transformations were monitored by an electrochemical quartz crystal nanobalance.
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