Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
17907 | Enzyme and Microbial Technology | 2007 | 7 Pages |
Abstract
The fermentation of the hydantoinase-active Pseudomonas putida RU-KM3s was investigated. The hydantoinase activity was found to be reduced in complex media, while the use of high sugar to nitrogen ratios in the growth medium resulted in the formation of extracellular polymers, low biomass, and low enzyme activity. By replacing the primary carbon source from simple carbohydrate to a fatty acid, using an ammonia-deficient medium (designated PP2 medium), a maximum growth rate of 0.29 (±0.005) hâ1 was attained with a biomass of 7.3 (±0.18) g Lâ1 at a yield of 0.73 g gâ1 and a productivity of 0.61 g gâ1 hâ1. The maximum hydantoinase activity obtained was 187 (±19.4) nmol minâ1 mgâ1 (DCW), while that of the N-carbamoylase was 22 (±3.1) nmol minâ1 mgâ1 (DCW). The fermentation was scaled up 50-fold from shake flask to 15 L batch.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
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Authors
Shaun Kirchmann, Petrus van Zyl, Dean Brady, Nasreen Abrahams, Sonia Rech, Rosemary Dorrington, Stephanie Burton,