| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 179264 | Electrochemistry Communications | 2013 | 4 Pages |
•An ultrasensitive electrochemical method is proposed for detection of microRNA-16.•Molecular beacon structure is designed to initiate RCA for the high selectivity.•To achieve signal amplification, RCA and quantum dots tagging are utilized.
MicroRNA-16 (miR-16) frequently deleted or downregulated in patients with B cell chronic lymphocytic leukemia and thus can be used as promising biomarkers in B-CLL diagnosis and prognosis. Herein, an ultrasensitive miR-16 detection was carried out by combining rolling circle amplification (RCA), quantum dots tagging and anodic stripping voltammetric detection (ASV). The method could specifically quantify miR-16 over a 6-decade dynamic range and reach an ultra-low detection limit of 0.32 aM.
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