Laccase cathode approaches to physiological conditions by local pH acidification

A new conceptual approach to improve the performance of a laccase-based cathode at neutral pH is presented. The working pH of Trametes hirsuta laccase, typically acidic, can be achieved by oxidation of biological compounds such as glucose catalyzed by a second enzyme immobilized in the vicinity of the laccase electrode.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Improvement of Trametes hirsuta laccase as biocathode catalyst under physiological conditions is presented. ► Electrode modified with laccase is socketed in a magnet. ► Acidifying magnetic nanoparticles are confined close to the biocathode. ► Local pH is acidified while keeping bulk pH neutral but changing it locally in the vicinity of the electrode. ► Oxygen electrorreduction by laccase is performed while keeping neutral bulk pH.