An electrochemiluminescence aptasensor for tumor cells assay based on signal amplification of Ru(II) covalently doped silica nanoparticles

A sensitive and stable aptasensor for the assay of tumor cells based on signal amplification with novel ruthenium(II) covalently doped silica nanoparticles (Si NPs) was presented for the first time. For the proposed aptasensor, one target signal could be transformed into multiple ECL signals of Ru(II) markers since a single Si NP could be loaded with multiple of Ru(II) markers. The binding of Si NPs on the electrode further increased the sensor sensitivity. The advantage of extraordinary stability of covalently doped Si NPs made the sensor exhibit excellent reproducibility. The new method for the assay of tumor cells is reliable.

► We have synthesized highly stable Ru(II) covalently doped Si NPs. ► An ECL aptasensor for tumor cells assay based on Si NPs is well developed. ► The novel sensor exhibits good sensitivity and excellent reproducibility. ► The linear range is from 100 to 2000 cells mL−1 for the detection of Ramos cells. ► The detection limit is down to 78 cells mL− 1.