Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
180394 | Electrochemistry Communications | 2010 | 5 Pages |
Abstract
For increasing the sensitivity of label-free DNA assays an amplification strategy is proposed based on the synthesis of a proflavine derivative which on the one hand retains its high affinity for double-stranded DNA (dsDNA) intercalation and on the other hand is functionalized via a flexible spacer with biotin moieties. By this, subsequent to the post-labeling of areas with dsDNA, reporter systems such as streptavidin/enzyme conjugates can be bound. Amplified DNA hybridization detection using an oligonucleotide model system, a biotinylated proflavine as intercalator and streptavidin/alkaline phosphatase is demonstrated.
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Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Magdalena Gębala, Leonard Stoica, Dmitrii Guschin, Lutz Stratmann, Gerhard Hartwich, Wolfgang Schuhmann,