Article ID Journal Published Year Pages File Type
180519 Electrochemistry Communications 2009 4 Pages PDF
Abstract

Electrochemical impedance probing of TATA binding protein (TBP) based on TATA box site-specific binding was described in this work. A sensitive detection of TBP was developed from TATA box DNA self assembly on the electrode and the impedance changes induced by TBP binding. Electrochemical impedance spectroscopy (EIS) probing of TBP had a sensitivity of 0.8 nM with excellent selectivity. Moreover, the interferences of triplex forming oligonucleotides (TFOs) and anticancer drug daunomycin on TBP binding to TATA box DNA were investigated by EIS. TFOs reduced the stability of TBP binding to TATA box, but daunomycin completely inhibited the TBP binding.

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Physical Sciences and Engineering Chemical Engineering Chemical Engineering (General)
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