Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
181657 | Electrochemistry Communications | 2008 | 4 Pages |
Direct electrochemistry of hemoglobin (Hb) has been achieved by its direct immobilization on carbon ionic liquid electrode (CILE). CILE was immersed in a solution containing Hb and ionic liquid, octylpyridinium chloride ([OcPy][Cl]), to directly immobilize Hb on CILE. Cyclic voltammetry of modified electrode exhibited quasi-reversible peaks corresponding to Hb. The oxidation and reduction peak potentials of immobilized Hb in acetate buffer solution, pH 5.0 and at a scan rate of 0.1 V s−1 were obtained at about –150 mV and –290 mV, respectively. The average surface coverage of the electroactive Hb adsorbed on the electrode surface was calculated as 8.4 × 10−11 mol cm−2. Hb retained its bioactivity on modified electrode and showed excellent electrocatalytic activity towards oxygen, hydrogen peroxide and nitrite. Hydrogen peroxide can be determined in the range of 1.0 × 10−4–5.0 × 10−3 M.