| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 18211 | Enzyme and Microbial Technology | 2006 | 4 Pages |
Abstract
Based on the effects of cadmium and copper on KlHIS4 transcription when expressed in either Kluyveromyces lactis or Saccharomyces cerevisiae, a yeast biosensor is presented. It consists of a lacZ fused to a KlHIS4 447 bp long promoter expressed in K. lactis, and as control, a second fusion with a shorter promoter not regulated by metals. This eukaryotic system can be used to detect cadmium and copper concentrations of 0.3–3 mM (or higher) within minutes using X-GAL filter assay and other variants of β-galactosidase assays developed in multititer wells.
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Authors
M.A. Freire-Picos, M. Lamas-Maceiras,