Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
18296 | Enzyme and Microbial Technology | 2007 | 5 Pages |
Abstract
Whole cells of mutated Xanthomonas campestris were successfully used as a biocatalyst for the glucosylation of kojic acid. Suitable carbon and nitrogen sources and the pH for the cultivation of the mutated cells at 30 °C were identified for maximum conversion. Using 0.1 g of the cells, 80% conversion was obtained after 28 h at 30 °C and by using maltose as the acyl donor at a molar ratio of 1:8. Under the same conditions, the use of wild type cells resulted in 13% conversion only. 1D NMR and 2D shift-correlated NMR (1H × 1H-COSY, 1H × 13C-COSY) analysis showed that the product was kojic acid 7-o-alpha-d-glucopyranoside.
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Authors
Hsin-Ju Hsieh, R. Giridhar, Wen-Teng Wu,