| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 18368 | Enzyme and Microbial Technology | 2007 | 6 Pages |
Abstract
A polymeric film consisting of two distinct monomers such as pyrrole–benzophenone and tris(bipyridine pyrrole) ruthenium (II) units was electrogenerated and used for the photochemical grafting of T7 bacteriophages displaying a specific West Nile virus (WNV) epitope. The resulting electrodes were applied to the amperometric detection of WNV antibody at 0 V in aqueous electrolyte via secondary peroxidase-labeled antibody, hydrogen peroxide and hydroquinone as the enzyme substrate. A calibration curve based on different WNV–antibody dilutions ranging from 10 to 106 was created with a relatively short response time of 30 s after substrate injection.
Related Topics
Physical Sciences and Engineering
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Bioengineering
Authors
Rodica E. Ionescu, Serge Cosnier, Gregoire Herzog, Karine Gorgy, Boaz Leshem, Sebastien Herrmann, Robert S. Marks,