Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
18554 | Enzyme and Microbial Technology | 2006 | 6 Pages |
Abstract
Saccharomyces cerevisiae invertase was chemically modified with chitosan and further immobilized on sodium alginate-coated chitin support. The yield of immobilized protein was determined as 85% and the enzyme retained 97% of the initial chitosan-invertase activity. The optimum temperature for invertase was increased by 10 °C and its thermostability was enhanced by about 9 °C after immobilization. The immobilized enzyme was stable against incubation in high ionic strength solutions and was four-fold more resistant to thermal treatment at 65 °C than the native counterpart. The biocatalyst prepared retained 80% of the original catalytic activity after 50 h under continuous operational regime in a packed bed reactor.
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Authors
Leissy Gómez, Hector L. Ramírez, Maria L. Villalonga, Junior Hernández, Reynaldo Villalonga,