Elimination of glucose in egg white using immobilized glucose oxidase

A commercial glucose oxidase containing catalase (NOVOZYM 771) was immobilized on Amberlite UP 900 anion exchange resin using adsorption and subsequent cross-linking with glutaraldehyde. The maximum protein loading was 33.0 mg g−1 dry support. The activity of the product was 10.2 units g−1 dry solid. The immobilized enzyme was used for the elimination of glucose from egg white in a pilot-scale three-stage fluidized-bed bioreactor with mechanical mixing, equipped with large porous surface for bubble-free transport of oxygen as co-substrate. The biocatalyst packing (10.9 dm3) was uniformly distributed between the stages. The best productivity was achieved using oxygen pressure of 1.5 bar and 2 h residence time at 25 °C.