Novel method of enzymes stabilization on crosslinked thermosensitive carriers

In this paper an immobilization of invertase on thermosensitive copolymers of N-isopropylacrylamide and 2-hydroxyethyl methacrylate or glycidyl methacrylate modified by aminolysis is evaluated. The method is based on the swelling properties of stimuli-sensitive polymers, which work like a pump that sucks up the enzyme on cooling and then on subsequent crosslinking of the enzyme. The attention was focused on the properties of the carrier–enzyme systems, particularly on the effect of crosslinking on their stability. Activity of TG8-NH2 carrier was very low and independent on concentration of glutaraldehyde used, but carriers TH8 and TH8-NH2 were more active, especially when 1.0 and 2.5 vol.% of glutaraldehyde were used. It was also observed, that preparations crosslinked by glutaraldehyde were more stable than preparations without crosslinking agent.