Evaluation of the conditions for the extraction of hydrolitic enzymes obtained by solid state fermentation from grape pomace

In this work, conditions for the extraction of xylanase and exo-poligalacturonase, previously produced by solid state fermentation on grape pomace using Aspergillus awamori, have been evaluated. The effect of the type and volume of solvent, temperature and time of incubation and stirring rate were studied separately and combined. Furthermore, the application of repetitive extractions was evaluated. Recommended conditions for both enzymes recovery from fermented grape pomace are 50 ml Tween 80 (0.01%), 4 °C and 150 rpm. In this way, xylanase and exo-PG recovery can be increased in 7.7- and 5.5-fold. In addition, the data are used as the base for the theoretical calculation of the total amount of enzyme which could be extracted from the solid.