Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1886808 | Radiation Physics and Chemistry | 2008 | 4 Pages |
Abstract
3-Nitrotyrosine(3-NY) has been reported as a potential source of reactive oxygen species (ROSs). In this work, plasmid pBR322 DNA was irradiated by gamma rays in aqueous solution in presence and absence of 3-NY, DNA strand breaks were analyzed by neutral electrophoresis followed by quantification with image analysis software. It was found that the presence of 3-NY could effectively reduce radiation-induced DNA strand breaks. A side-by-side comparison was performed between 3-NY and tyrosine, the results showed that the protective role 3-NY was comparable with tyrosine, which might imply that protein tyrosine nitration might not significantly decrease its ability as a free radical scavenger.
Related Topics
Physical Sciences and Engineering
Physics and Astronomy
Radiation
Authors
Wei-Qun Shi, Mei-Nan Ni, Fu-Quan Kong, Li Sui, Jia Hu, Dian-Dou Xu, Yan-Mei Li,