| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1923359 | Acta Histochemica | 2015 | 5 Pages |
•We analyzed the expression patterns and localization of HGF and its receptor in skin of white versus brown alpaca.•HGF staining showed strongly positive in the dermal papilla and mesenchymal cells of white alpaca skin.•c-Met staining showed strongly positive in hair matrix and root sheath of brown alpaca skin.•The protein and transcripts possessed the same expression pattern in white and brown alpaca skin.•The results offer theoretical basis for exploration on the role of HGF/c-Met signaling in alpaca.
Hepatocyte growth factor (HGF)/c-Met signaling has been considered as a key pathway in both melanocyte development and melanogenesis. To understand better the expression patterns and tissue distribution characterization of HGF and its receptor c-Met in skin of white versus brown alpaca (Vicugna pacos), we detected the tissue distribution of HGF and c-Met using immunohistochemistry and analyzed the expression patterns by using Western blot and quantitative real time PCR (qPCR). Immunohistochemistry analysis demonstrated that HGF staining robustly increased in the dermal papilla and mesenchymal cells of white alpaca skin compared with that of brown. However, c-Met staining showed strongly positive result, particularly inhair matrix and root sheath in brown alpaca skin. Western blot and qPCR results suggested that HGF and c-Met were expressed at significantly high levels in white and brown alpaca skins, respectively, and protein and transcripts possessed the same expression pattern in white and brown alpaca skins. The results suggested that HGF/c-Met signaling functions in alpaca coat color formation offer essential theoretical basis for further exploration of the role of HGF/c-Met signaling in pigment formation.
