Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1924315 | Acta Histochemica | 2007 | 10 Pages |
Abstract
Both cell lines bound to DBA-derivatized microbeads, and binding was inhibited by N-acetyl-d-galactosamine, but not by l-fucose. Neither cell line bound to PNA-derivatized microbeads. Despite the lack of lectin binding using the rapid microbead method, PNA was mitogenic in culture at some time points and its mitogenic effect displayed a reverse-dose response. This was also seen with effects of DBA on cells in culture. While this is a simple study, the results were statistically highly significant and suggest that: (1) agents may not need to bind strongly to cells to exert biological effects, (2) cell line pairs derived from diseased and non-diseased tissue can provide useful comparative data on potential drug effects and (3) very low concentrations of potential drugs might be initially tested experimentally because reverse-dose responses should be considered.
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Authors
Karineh Petrossian, Lisa R. Banner, Steven B. Oppenheimer,