Article ID Journal Published Year Pages File Type
1925361 Archives of Biochemistry and Biophysics 2013 9 Pages PDF
Abstract

- RS223-BX is strongly activated by Ca2+, Co2+, Fe2+, Mg2+, Mn2+ and Ni2+.
- The β-xylosidase is inactivated by Cu2+ and Zn2+.
- E binds Mg2+, E Mg2+ isomerizes slowly to E∗ Mg2+ and E∗ Mg2+ binds substrate.
- Metal-activated RS223-BX exhibits the highest known kcat/Km on xylooligosaccharides.
- RS223-BX has potential use in the saccharification of xylan for fermentation purposes.
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