| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1925691 | Archives of Biochemistry and Biophysics | 2011 | 8 Pages |
Abstract
⺠Hyperthermophilic chaperonins (Cpn) are highly stable yet conserved with other archaeal HSP60s. ⺠The C-termini contain highly charged sections, divergent from mesophilic archaeal Cpns. ⺠Mutation of the thermophile domain to reduce charge produced “cold-adapted” (Topt 40 °C) variants. ⺠Mutants with depressed Topt showed enhanced folding of mesophilic enzymes. ⺠Raised optimal temperature in Cpn from a psychrophile by chimera formation with the C-terminus of hyperthermophilic Cpn60.
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Authors
Haibin Luo, Frank T. Robb,