Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1926142 | Archives of Biochemistry and Biophysics | 2010 | 6 Pages |
Abstract
Bioactive compounds of great interest are found in the saliva of hematophagous organisms. While exploring a cDNA library derived from the salivary glands of the tick Amblyommacajennense, a transcript that codes for a protein with unique structure (containing an N-terminal Kunitz-type domain and a C-terminus with no homology to any annotated sequences) was found. The recombinant mature form of this protein (∼13.5 kDa) was produced in Escherichia coli BL21 (DE3), and it was able to inhibit Factor Xa (FXa) and extend global blood clotting times in vitro and ex vivo. Static and dynamic predictions of its tertiary structure indicate regions that may be related to its FXa inhibitor function.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
I.F.C. Batista, O.H.P. Ramos, J.S. Ventura, I.L.M. Junqueira-de-Azevedo, P.L. Ho, A.M. Chudzinski-Tavassi,