Article ID Journal Published Year Pages File Type
1926870 Archives of Biochemistry and Biophysics 2007 6 Pages PDF
Abstract

To investigate the signal transduction pathway of caspase-2, cell permeable Tat–reverse-caspase-2 was constructed, characterized and utilized for biochemical and cellular studies. It could induce the cell death as early as 2 h, and caspase-2-specific VDVADase activity but not other caspase activities including DEVDase and IETDase. Interestingly, nuclear DNA fragmentation occurred and consistently DNA fragmentation factor (DFF45)/Inhibitor of caspase-activated DNase (ICAD) was cleaved inside the cell as well as in vitro, suggesting a role of caspase-2 in nuclear DNA fragmentation.

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