| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1927021 | Archives of Biochemistry and Biophysics | 2007 | 8 Pages |
Abstract
The influence of the promoter and an N-terminal hexahistidine tag on human cardiac actin (ACTC) expression and function was investigated using four baculovirus constructs. It was found that both non-tagged ACTC and hisACTC expression from the p10 promoter was higher than from the polh promoter. Characterization showed that an N-terminal hexahistidine tag has a negative effect on ACTC. Recombinant ACTC inhibits DNase-I and binds myosin S1, indicative of proper folding. Our data support the hypothesis that the actin protein down-regulates the polh promoter.
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Authors
Susan P. Yates, Marissa D. Otley, John F. Dawson,