Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1928041 | Biochemical and Biophysical Research Communications | 2015 | 6 Pages |
Abstract
2,2,2-Trichloroethanol (TCOH) is responsible for the pharmacological actions of chloral hydrate (CH), and is a major metabolite of trichloroethylene. Human exposure to TCOH is known to be increasing. Recently, it was reported that TCOH causes a significant phase delay of Per2 expression in mouse liver when injected daily over the course of several days. However, it is not clear whether TCOH directly modulates the molecular clock. In the present study we used a cell-based assay system to test this possibility. We found that the daily oscillation period of Bmal1 was lengthened to 3Â h following treatment with 1.5Â mM TCOH, and increased to 5Â h with 3Â mM TCOH treatment. However, low concentrations of TCOH had no noticeable effects. The effect of TCOH on Per2 oscillation was marginal. Interestingly, serum from rats anesthetized with CH also modulated Bmal1 period, suggesting that exposure to anesthesia should be taken into consideration for circadian rhythm studies. In summary, our study reveals a direct regulation of TCOH on molecular clock.
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Authors
Jie Gao, Caixia Yang, Hui Ding, Yanli Zhang, Jinhua Xue, Yanning Cai,