| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1928402 | Biochemical and Biophysical Research Communications | 2014 | 7 Pages |
•Construct and characterization of the noncovalently assembled ZFN were carried out.•The coiled-coil interaction (leucine zippers) is used as a noncovalent assembler.•ncZFNs are fully functional and sequence-specific like conventional ZFN.•The source of augmented off-target cleavage of ncZFN was investigated.•We demonstrate creation of pseudo-multidomain proteins assembled by leucine zippers.
Zinc finger nuclease (ZFN) is a chimeric restriction enzyme made of a C2H2-type zinc finger protein (ZFP) and the FokI nuclease domain (FN). ZFN technology has been considered as a powerful tool for genome editing. Here, we report a new type of ZFN system based on the coiled-coil interaction used as a noncovalent assembler. Like conventional ZFNs, noncovalently assembled ZFNs (ncZFNs) structurally have two domains, a ZFP and a FN. Each domain carries one of antiparallel heterodimeric leucine zippers, respectively, to form an ncZFN through leucine zipper assembly. The characterization of ncZFNs revealed that they behave as fully functional sequence-specific endonucleases, comparable to those of conventional ZFNs. Interestingly, some ncZFNs displayed augmented off-target cleavage, possibly by degenerate DNA binding of the ZFP domain of ncZFNs based on our data. We postulate that DNA cleavage of ncZFN(s) seems to be more sensitive to the ZFP binding to lesser-optimal sites. Facile design of ncZFNs through the mix-and-assemble approach could be applicable to other DNA binding proteins for evaluation of sequence-specificity. In addition, our work establishes that the coiled-coil interaction could be used as the peptide-based noncovalent assembler for the formation of a noncovalently-linked functional multidomain protein.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide
