Article ID Journal Published Year Pages File Type
1928448 Biochemical and Biophysical Research Communications 2014 7 Pages PDF
Abstract

•Pre-treatment with the inhibitors increased the sensitivity of Jurkat cells to irradiation.•Combining both inhibitors together resulted in a G2 cell cycle arrest abrogation in Jurkat.•Jurkat cells pre-treated with inhibitors were positive for γH2AX foci 24 h upon irradiation.•Pre-treatment with Rad51 RI-1 had no effect on apoptosis induction in MOLT-4 cells.•When dosed together, the combination decreased MOLT-4 cell survival.

Present-day oncology sees at least two-thirds of cancer patients receiving radiation therapy as a part of their anticancer treatment. The objectives of the current study were to investigate the effects of the small molecule inhibitors of Wee1 kinase II (681641) and Rad51 (RI-1) on cell cycle progression, DNA double-strand breaks repair and apoptosis following ionizing radiation exposure in human leukemic T-cells Jurkat and MOLT-4. Pre-treatment with the Wee1 681641 or Rad51 RI-1 inhibitor alone increased the sensitivity of Jurkat cells to irradiation, however combining both inhibitors together resulted in a further enhancement of apoptosis. Jurkat cells pre-treated with inhibitors were positive for γH2AX foci 24 h upon irradiation. MOLT-4 cells were less affected by inhibitors application prior to ionizing radiation exposure. Pre-treatment with Rad51 RI-1 had no effect on apoptosis induction; however Wee1 681641 increased ionizing radiation-induced cell death in MOLT-4 cells.

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Authors
, , , , , , , , ,