| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1928623 | Biochemical and Biophysical Research Communications | 2014 | 5 Pages |
•Id1-b inhibits TGF-β-induced collagen expression in dermal fibroblasts.•Id1-b inhibits TGF-β-induced phosphorylation of Smad2 and Smad3.•Id1 expression was decreased in fibrotic skin diseases while TGF-β signaling was increased.
Inhibitor of DNA binding 1 (Id1) is a basic helix-loop-helix (bHLH) protein that has a variety of functional roles in cellular events including differentiation, cell cycle and cancer development. In addition, it has been demonstrated that Id1 is related with TGF-β and Smad signaling in various biological conditions. In this study, we investigated the effect of Id1 on TGF-β-induced collagen expression in human dermal fibroblasts. When Id1-b isoform was overexpressed, TGF-β-induced collagen expression was markedly inhibited. Consistent with this result, Id1-b significantly inhibited TGF-β-induced collagen gel contraction. In addition, Id1-b inhibited TGF-β-induced phosphorylation of Smad2 and Smad3. Finally, immunohistochemistry showed that Id1 expression was decreased in fibrotic skin diseases while TGF-β signaling was increased. Together, these results suggest that Id1 is an inhibitory regulator on TGF-β-induced collagen expression in dermal fibroblasts.
