Article ID Journal Published Year Pages File Type
1928857 Biochemical and Biophysical Research Communications 2013 8 Pages PDF
Abstract

•Quantitative proteomics was applied to the lateral amygdala.•Proteome-wide changes upon auditory fear conditioning and unpairing were characterized.•Various proteins previously implicated in LTP and learning were regulated by learning.•A substantial number of novel uncharacterized proteins were also regulated.

Auditory fear conditioning is a well-characterized rodent learning model where a neutral auditory cue is paired with an aversive outcome to induce associative fear memory. The storage of long-term auditory fear memory requires long-term potentiation (LTP) in the lateral amygdala and de novo protein synthesis. Although many studies focused on individual proteins have shown their contribution to LTP and fear conditioning, non-biased genome-wide studies have only recently been possible with microarrays, which nevertheless fall short of measuring changes at the level of proteins. Here we employed quantitative proteomics to examine the expression of hundreds of proteins in the lateral amygdala in response to auditory fear conditioning. We found that various proteins previously implicated in LTP, learning and axon/dendrite growth were regulated by fear conditioning. A substantial number of proteins that were regulated by fear conditioning have not yet been studied specifically in learning or synaptic plasticity.

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Authors
, , , , , , , , , , , , , , ,