| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1928961 | Biochemical and Biophysical Research Communications | 2013 | 6 Pages |
As the first and main form of active immune defense in the central nervous system, microglial cells usually exhibit complicated intracellular calcium (Ca2+) activity that can regulate the downstream components of signaling cascades. In the present work, spontaneous oscillations of the cytosolic calcium concentration ([Ca2+]c) in multi-BV-2 microglial cells were observed by video microscopy. These cells exhibited random spikes of Ca2+ oscillations. Cross-correlation analysis of the temporal dependence of the oscillations indicated the existence of cell–cell communication mediated by extracellular messengers. Numerical simulations based on a simple mathematical model suggested that these communications could induce random spikes of spontaneous Ca2+ oscillations in the multi-cell system. Short-time imaging analysis of random spikes in different regions of a single cell showed that spontaneous Ca2+ oscillations resulted from Ca2+ wave generated by other cells as well as from calcium elevation inside the cell. Taken together, our data demonstrate that cell–cell communication existed between the BV-2 microglial cells in vitro and further resulted in the random spikes of spontaneous Ca2+ oscillations.
► Cross-correlation analysis suggests cell–cell communication exists between BV-2 cells. ► Random spikes of spontaneous Ca2+ oscillations are simulated by introducing random β. ► Spontaneous Ca2+ oscillations result from Ca2+ waves and global Ca2+ elevation. ► Cell–cell communication induces random spikes of spontaneous Ca2+ oscillations.
