Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1929305 | Biochemical and Biophysical Research Communications | 2012 | 6 Pages |
We report herein the functional expression of an Fe-type nitrile hydratase (NHase) without the co-expression of an activator protein or the Escherichia coli chaperone proteins GroES/EL. Soluble protein was obtained when the α- and β-subunit genes of the Fe-type NHase Comamonas testosteroni Ni1 (CtNHase) were synthesized with optimized E. coli codon usage and co-expressed. As a control, the Fe-type NHase from Rhodococcus equi TG328–2 (ReNHase) was expressed with (ReNHase+Act) and without (ReNHase−Act) its activator protein, establishing that expression of a fully functional, metallated ReNHase enzyme requires the co-expression of its activator protein, similar to all other Fe-type NHase enzymes reported to date, whereas the CtNHase does not. The X-ray crystal structure of CtNHase was determined to 2.4 Å resolution revealing an αβ heterodimer, similar to other Fe-type NHase enzymes, except for two important differences. First, two His residues reside in the CtNHase active site that are not observed in other Fe-type NHase enzymes and second, the active site Fe(III) ion resides at the bottom of a wide solvent exposed channel. The solvent exposed active site, along with the two active site histidine residues, are hypothesized to play a role in iron incorporation in the absence of an activator protein.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Functional expression of an Fe-type nitrile hydratase without an activator protein. ► X-ray structure of the nitrile hydratase from Comamonas testosteroni Ni1 at 2.4 Å. ► Iron active site resides at the bottom of a wide solvent exposed channel. ► Two His residues reside in the active site that are not conserved. ► Solvent exposed active site and/or His residues may play a role in iron incorporation.