Article ID Journal Published Year Pages File Type
1929466 Biochemical and Biophysical Research Communications 2012 4 Pages PDF
Abstract

Mutations in ALS2 gene/alsin are associated with recessive forms of motor neuron disorders including Juvenile Amyotrophic Lateral Sclerosis (JALS), Infantile-onset Ascending Hereditary Spastic Paraplegia (IAHSP) and Juvenile Primary Lateral Sclerosis (JPLS). In this study, we show that alsin and another MND-linked protein, spartin are related to each other both at mRNA and protein levels in Neuro2a cells. We observed significant alterations in spartin expression in alsin knock-down conditions. We further found that both proteins colocalize in N2a cells and spartin isoform-a precipitates with alsin in the same protein complex. In the light of these results we suggest that alsin and spartin may interact each other physically.

► In alsin KD cells, Spg20 mRNA levels are significantly altered. ► Alsin and spartin proteins colocalize and coprecipitate in N2a cells. ► Alsin and spartin may physically interact, having related functions in EGFR trafficking.

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