Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1929719 | Biochemical and Biophysical Research Communications | 2012 | 5 Pages |
Abstract
⺠mOCTN3 transport protein has been cloned in pET-21a(+) and over-expressed in Escherichia coli. ⺠The expressed mOCTN3 has been purified to homogeneity by Ni-chelating chromatography. ⺠The protein solubilised in Triton X-100 has been reconstituted in liposomes. ⺠Recombinant mOCTN3 catalyses transport of carnitine by a uniport mode.
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Authors
Mariafrancesca Scalise, Michele Galluccio, Lorena Pochini, Cesare Indiveri,