Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1930005 | Biochemical and Biophysical Research Communications | 2011 | 6 Pages |
Abstract
⺠aIF5B forms maximum 5.0-6.8% irreversible dimers in solution. ⺠Sedimentation coefficients for monomer and dimer are 3.64 and 5.51 ± 0.29 S. ⺠Adding only 2% glycerol prevents dimerization. ⺠SAXS on aIF5B monomer gave an Rg of 37.5 ± 0.2 Ã
and a Dmax of â¼130Â Ã
. ⺠There are universal structural differences between aIF5B and Escherichia coli IF2.
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Authors
Louise Carøe Vohlander Rasmussen, Cristiano Luis Pinto Oliveira, Olwyn Byron, Janni Mosgaard Jensen, Jan Skov Pedersen, Hans Uffe Sperling-Petersen, Kim Kusk Mortensen,