Article ID Journal Published Year Pages File Type
1930047 Biochemical and Biophysical Research Communications 2012 5 Pages PDF
Abstract

Chlamydomonas reinhardtii is a model species of algae for studies on the circadian clock. Previously, we isolated a series of mutants showing defects in the circadian rhythm of a luciferase reporter introduced into the chloroplast genome, and identified the genes responsible for the defective circadian rhythm. However, we were unable to identify the gene responsible for the defective circadian rhythm of the rhythm of chloroplast 97 (roc97) mutant because of a large genomic deletion. Here, we identified the gene responsible for the roc97 mutation through a genetic complementation study. This gene encodes a protein that is homologous to the subunit of N-terminal acetyltransferase (NAT) which catalyzes N-terminal acetylation of proteins. Our results provide the first example of involvement of the protein N-terminal acetyltransferase in the circadian rhythm.

► roc97 is a circadian rhythm mutant of the green alga Chlamydomonas reinhardtii. ► We identified the gene responsible for the roc97 mutation. ► The gene encodes a subunit of the protein N-terminal acetyltransferase (NAT).

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Authors
, , ,