| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1930127 | Biochemical and Biophysical Research Communications | 2012 | 6 Pages |
Endothelial outgrowth cells (EOCs) derived from blood mononuclear cells can differentiate to an endothelial-like phenotype. There are deficits in understanding of the biology of these cells, particularly detailed characterisation of their Ca2+ signalling mechanisms. In the current study, it was found that human EOCs express two forms of ryanodine receptor (RyR1 and RyR2) Ca2+ release channel in their endoplasmic reticulum. Individual EOCs display heterogeneous Ca2+ responses to physiologically relevant regulators fibrinogen and collagen. Some EOCs showed distinctive, multiphasic Ca2+ responses to fibrinogen consisting of rapid decreases, transient increases then a gradual return to the resting levels. Transient elevations in Ca2+ required both L-type voltage gated calcium channels and RyRs. Decreases in Ca2+ stimulated by fibrinogen depended on plasma membrane Ca2+ ATPase pumps, but did not require thapsigargin-sensitive Ca2+ ATPases. These results indicate that EOCs possess sophisticated Ca2+ signalling mechanisms, capable of generating distinct Ca2+ waveforms in response to different physiologically relevant cues.
► Endothelial outgrowth cells (EOCs) express ryanodine receptors and voltage gated calcium channels. ► Individual cells in an EOC population display heterogeneous calcium responses to fibrinogen or collagen. ► Increases in Ca2+ in response to fibrinogen involve ryanodine receptors and voltage gated calcium channels. ► Decreases in Ca2+ in response to fibrinogen are due to plasma membrane calcium ATPases.
