| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1930353 | Biochemical and Biophysical Research Communications | 2011 | 5 Pages |
The aim of this study was to search for a good inducer agent using for cardiomyogenic differentiation of stem cells. Human placenta-derived mesenchymal stem cells (hPDMSCs) were isolated and incubated in enriched medium. Fourth passaged cells were treated with 10 mg/L dan-shen root for 20 days. Morphologic characteristics were analyzed by confocal and electron microscopy. Expression of α-sarcomeric actin was analyzed by immunohistochemistry. Expression of cardiac troponin-I (TnI) was analyzed by immunohistofluorescence. Atrial natriuretic factor (ANF) and beta-myocin heavy chain (β-MHC) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). hPDMSCs treated with dan-shen root gradually formed a stick-like morphology and connected with adjoining cells. On the 20th day, most of the induced cells stained positive with α-sarcomeric actin and TnI antibody. ANF and β-MHC were also detected in the induced cells. Approximately 80% of the cells were successfully transdifferentiated into cardiomyocytes. In conclusion, dan-shen root is a good inducer agent used for cardiomyogenic differentiation of hPDMSCs.
► Conditional medium and dan-shen root were used for cardiomyogenic differentiation. ► They all could induce hPDMSCs to differentiate into cardiomyocytes. ► The induction effect of the latter was slightly higher compared to that of the former. ► Dan-shen root could be a good inducer for cardiomyogenic differentiation.
