Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1930413 | Biochemical and Biophysical Research Communications | 2011 | 5 Pages |
Apoptotic cell death eventually results in secondary necrotic cell death, whereas caspase-independent primary necrotic cell death has been reported and its mechanism involving RIP1 and RIP3 has been recently elucidated. Dual staining with fluorescent Annexin V and propidium iodide (PI) has been used to discriminate apoptotic and necrotic cell death, in which Annexin V-positive/PI-negative staining is regarded as apoptosis and PI-positive staining as necrosis. Here we demonstrate that primary necrotic cells unexpectedly show Annexin V-positive/PI-negative staining before they become PI-positive, and that primary necrotic and apoptotic Annexin V-positive/PI-negative cells can be discriminated by necrostatin-1, an inhibitor of primary necrosis by inhibition of RIP1.
► Annexin V-positive/PI-negative staining is regarded as apoptosis and PI-positive staining as necrosis. ► Primary necrotic cells unexpectedly show Annexin V-positive/PI-negative staining before they become PI-positive. ► Primary necrotic and apoptotic Annexin V-positive/PI-negative cells can be discriminated by necrostatin-1.