| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1930518 | Biochemical and Biophysical Research Communications | 2011 | 5 Pages |
Abstract
⺠A polypeptide, C-CPE, is a claudin-4 binder. ⺠We tried to prepare novel claudin-4 binders using C-CPE as a prototype. ⺠Preparation of C-CPE mutants based on the functional domain mapping. ⺠We got C-CPE mutants with 23.9-fold affinity to claudin-4 than C-CPE.
Keywords
C-CPEPBSCPEtransepithelial electric resistanceDDMTEERTBSSite-directed mutagenesisn-Dodecyl-β-D-maltosideTight junctionsodium dodecyl sulfate–polyacrylamide gel electrophoresisSDS-PAGEClostridium perfringens enterotoxinTris-buffered salineELISAEnzyme-linked immunosorbent assaySurface plasmon resonanceSPRPhosphate-buffered salinepolymerase chain reactionPCRclaudin
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Authors
Azusa Takahashi, Masuo Kondoh, Hiroshi Uchida, Yohei Kakamu, Takao Hamakubo, Kiyohito Yagi,