Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1931173 | Biochemical and Biophysical Research Communications | 2011 | 6 Pages |
Abstract
Yeast is a model eukaryote with a variety of biological resources. Here we developed a method to track a quantum dot (QD)-conjugated protein in the budding yeast Saccharomyces cerevisiae. We chemically conjugated QDs with the yeast prion Sup35, incorporated them into yeast spheroplasts, and tracked the motions by conventional two-dimensional or three-dimensional tracking microscopy. The method paves the way toward the individual tracking of proteins of interest inside living yeast cells.
Research highlights► We develop a method to track a quantum dot-conjugated protein in yeast cells. ► We incorporate the conjugated quantum dot proteins into yeast spheroplasts. ► We track the motions by conventional or 3D tracking microscopy.
Keywords
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Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Toshikazu Tsuji, Shigeko Kawai-Noma, Chan-Gi Pack, Hideki Terajima, Junichiro Yajima, Takayuki Nishizaka, Masataka Kinjo, Hideki Taguchi,