Article ID Journal Published Year Pages File Type
1931232 Biochemical and Biophysical Research Communications 2010 5 Pages PDF
Abstract

The hypothesis, that structural deterioration of cytochrome c oxidase (CcO) is a causal factor in the age-related decline in mitochondrial respiratory activity and an increase in H2O2 generation, was tested in Drosophila melanogaster. CcO activity and the levels of seven different nuclear DNA-encoded CcO subunits were determined at three different stages of adult life, namely, young-, middle-, and old-age. CcO activity declined progressively with age by 33%. Western blot analysis, using antibodies specific to Drosophila CcO subunits IV, Va, Vb, VIb, VIc, VIIc, and VIII, indicated that the abundance these polypeptides decreased, ranging from 11% to 40%, during aging. These and previous results suggest that CcO is a specific intra-mitochondrial site of age-related deterioration, which may have a broad impact on mitochondrial physiology.

Research highlights► Cytochrome c oxidase loses catalytic activity during the aging process. ► Abundance of seven nuclear-encoded subunits of cytochrome c oxidase decreased with age in Drosophila. ► Cytochrome c oxidase is specific intra-mitochondrial site of age-related deterioration.

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Authors
, , , , ,