| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1931277 | Biochemical and Biophysical Research Communications | 2011 | 5 Pages |
Apolipoprotein C-I (apoC-I) is a 6.6 kDa serum protein associated with high density lipoproteins (HDL) and triglyceride-rich lipoproteins. In this study, apoC-I was examined in high density lipoprotein subfractions from individuals with and without coronary artery disease (CAD). New isoforms of apoC-I, were detected in the cohort of individuals with CAD using mass spectrometry while the expected apoC-I isoforms were absent. In addition, the apoC-I mass spectra for the CAD cohort had satellite peaks indicative of the involvement of oxidative processes. Further analysis of the mass spectra of the CAD and non-CAD cohorts suggest that the origin of these new isoforms may be due to genetic mutations that could compromise the function of apoC-I.
Research highlights► Isoforms of apoC-I detected in HDL subfractions from individuals with coronary artery disease (CAD) that were not detected in the non-CAD cohort. ► m/z shifting of apoC-I isoform peaks observed at approximately +90 Da in all individuals with CAD. ► Further modifications observed in the apoC-I spectra of some individuals with CAD which showed doublet apoC-I peaks with mass differences indicative of oxidation. ► Findings contribute to the understanding of the unique functional properties of HDL.
