Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1931365 | Biochemical and Biophysical Research Communications | 2010 | 5 Pages |
Cell-free translation is one potential approach to the production of functional transmembrane proteins. We have now examined various detergents as supplements to a wheat-germ cell-free system in order to optimize the production and subsequent purification of a functional model transmembrane protein, bacteriorhodopsin. We found that Fos-choline and CHAPS detergents counteracted each other’s inhibitory effects on cell-free translation activity and thereby allowed the efficient production and subsequent purification of functional bacteriorhodopsin in high yield.
Research highlights► Efficient production of functional bacteriorhodopsin with a wheat-germ cell-free system. ► Finding of unique combination of detergents, Fos-choline and CHAPS. ► The detergent mix is compatible with a procedure for the protein purification.