Article ID Journal Published Year Pages File Type
1931365 Biochemical and Biophysical Research Communications 2010 5 Pages PDF
Abstract

Cell-free translation is one potential approach to the production of functional transmembrane proteins. We have now examined various detergents as supplements to a wheat-germ cell-free system in order to optimize the production and subsequent purification of a functional model transmembrane protein, bacteriorhodopsin. We found that Fos-choline and CHAPS detergents counteracted each other’s inhibitory effects on cell-free translation activity and thereby allowed the efficient production and subsequent purification of functional bacteriorhodopsin in high yield.

Research highlights► Efficient production of functional bacteriorhodopsin with a wheat-germ cell-free system. ► Finding of unique combination of detergents, Fos-choline and CHAPS. ► The detergent mix is compatible with a procedure for the protein purification.

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Authors
, , ,