The role of iron in the proliferation of Drosophila l(2)mbn cells

Iron is essential for life and is needed for cell proliferation and cell cycle progression. Iron deprivation results first in cell cycle arrest and then in apoptosis. The Drosophila tumorous larval hemocyte cell line l(2)mbn was used to study the sensitivity and cellular response to iron deprivation through the chelator desferrioxamine (DFO). At a concentration of 10 μM DFO or more the proliferation was inhibited reversibly, while the amount of dead cells did not increase. FACS analysis showed that the cell cycle was arrested in G1/S-phase and the transcript level of cycE was decreased to less than 50% of control cells. These results show that iron chelation in this insect tumorous cell line causes a specific and coordinated cell cycle arrest.

Research highlights► Establishment of a model system to study the role of iron during proliferation. ► Iron deprivation of insect tumorous cell line inhibits cell proliferation. ► Iron deprivation causes a reversible cell cycle arrest in G1/S-phase. ► Iron deprivation promotes decreased gene expression of cycE.