Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1932064 | Biochemical and Biophysical Research Communications | 2010 | 5 Pages |
Abstract
miR-301 is localized in the first intron of ska2, whose function has not been clarified. Here, a new circuit model in which intronic miR-301 regulates the transcription and function of its host gene through a feedback mechanism has been described. Our results showed that blocking of miR-301 in A549 cells leads to a decrease in the expression of the host gene, ska2. Further analysis showed that miR-301 targets MEOX2 to affect the ERK/CREB pathway. CREB directly regulates the expression of the host gene, ska2. In addition, the inhibition of miR-301 or ska2 resulted in an increase of the mitotic index and a decrease in colony formation in soft agar, which may be related to lung tumorigenesis.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Guojun Cao, Baochun Huang, Zhaohui Liu, Jiyan Zhang, Hua Xu, Wei Xia, Jie Li, Shaohua Li, Liucun Chen, Hongmei Ding, Qiang Zhao, Ming Fan, Beifen Shen, Ningsheng Shao,