Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1932123 | Biochemical and Biophysical Research Communications | 2010 | 4 Pages |
Abstract
We immobilize α-amylase extracted from Bacillus Iicheniformis on the surfaces of superparamagnetic particles and investigate the effect of a rotational magnetic field on the enzyme’s activity. We find that the activity of the enzyme molecules immobilized on superparamagnetic particles increases in the rotational magnetic field and reaches maximum at a certain frequency. We clarify the effect of the cluster structures formed by the superparamagnetic particles on the activity. Enzyme reactions are enhanced even in a tiny volume of solution using the present method, which is very important for the development of efficient micro reactors and micro total analysis systems (μ-TAS).
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Authors
Toru Mizuki, Noriyuki Watanabe, Yutaka Nagaoka, Tadamasa Fukushima, Hisao Morimoto, Ron Usami, Toru Maekawa,