Lipids modulate H2S/HS− induced NO release from S-nitrosoglutathione

Recently we observed that a gas messenger H2S/HS− released NO from S-nitrosoglutathione (Ondrias et al., Pflugers Arch. 457 (2008) 271–279). However, the effect of biological compounds on the release is not known. Measuring the NO oxidation product, which is nitrite, by the Griess reaction, we report that unsaturated fatty acid—linoleic acid and lipids having unsaturated fatty acids: asolectin, dioleoylphosphocholine and dioleoylphosphoserine depressed the H2S/HS− induced NO release from S-nitrosoglutathione. On the other hand, a depression effect of the saturated fatty acid—myristic acid and lipids having saturated fatty acids, dilauroylphosphatidylcholine, dimyristoylphosphatidylcholine, dipalmitoylphosphatidylcholine and distearoylphosphatidylcholine was less pronounced. The inhibition effect increased with the decreasing gel-to-liquid phase transitions temperature of the fatty acids and lipids. We suggest that lipid composition of biological membranes modulates NO release from nitrosoglutathione induced by H2S/HS−, assuming that a reaction of H2S/HS− with unsaturated bonds of fatty acids may be partially responsible for the effect.