Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1933848 | Biochemical and Biophysical Research Communications | 2009 | 5 Pages |
Abstract
During the screening for novel differentiation inducers, we found that a culture broth of Streptomyces sp. HK-803 induced myeloid differentiation of HL-60 cells. The active substance was identified as deamino-hydroxy-phoslactomycin B (HPLM) by mass spectrometry, and synthesized HPLM also induced the differentiation of HL-60 cells. HPLM showed greater inhibition of protein phosphatase 2A (PP2A) activity than phoslactomycin B (PLMB); however, PLMB and okadaic acid did not induce differentiation. Moreover, treatment with ATRA and 1α, 25(OH)2D3 induced retinoic acid receptor-β and 1α, 25(OH)2 D3 24-hydroxylase, respectively, whereas HPLM did not, suggesting that HPLM is a novel differentiation inducer.
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Authors
Siro Simizu, Takayuki Teruya, Toshihiko Nogawa, Harumi Aono, Masashi Ueki, Masakazu Uramoto, Yuichi Kobayashi, Hiroyuki Osada,