Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1934577 | Biochemical and Biophysical Research Communications | 2009 | 5 Pages |
Abstract
Constitutive upregulation and a higher degree of induction of drug metabolism and disposition-related genes were found in a three-dimensional HepG2 culture. The upregulated genes are believed to be regulated by different regulatory factors. Global gene expression analysis using the Affymetrix GeneChip indicated that altered expression of microtubule-related genes may change the expressed levels of drug metabolizing and disposition genes. Stabilization of microtubule molecules with docetaxel, a tubulin-stabilizing agent, in the two-dimensional culture showed gene expression patterns similar to those found in the three-dimensional culture, indicating that the culture environment affects drug metabolism functions in HepG2 cells.
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Authors
Shin-ichiro Horiuchi, Seiichi Ishida, Tomokatsu Hongo, Youichi Ishikawa, Atsuko Miyajima, Jun-ichi Sawada, Yasuo Ohno, Ken Nakazawa, Shogo Ozawa,