Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1934610 | Biochemical and Biophysical Research Communications | 2008 | 6 Pages |
Abstract
Reciprocal functional inhibition between P2X and GABAA/C receptors represents a novel mechanism fine-tuning neuronal excitability. However, the participating receptors and underlying mechanisms are not fully understood. P2X4 receptor is widely found in neurons that express GABAC Ï1 receptor. Thus, we co-expressed P2X4 and Ï1 receptors in HEK293 cells and, using patch-clamp recording, examined whether they have mutual functional inhibition. Currents evoked by simultaneous application of ATP and GABA (IATP+GABA) were significantly smaller compared to the addition of IATP and IGABA. Furthermore, IATP were strongly suppressed during Ï1 receptor activation. Similarly, IGABA were greatly attenuated during P2X4 receptor activation. Such mutual inhibition was absent in cells only expressing P2X4 or Ï1 receptor. Taken together, these functional data support negative cross-talk between P2X4 and Ï1 receptors.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Rong Xia, Zhu-Zhong Mei, Carol Milligan, Lin-Hua Jiang,