Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1934720 | Biochemical and Biophysical Research Communications | 2009 | 6 Pages |
Abstract
Structure-switching signaling aptamers are nucleic acids that change shape upon binding to a specific ligand. Previously, we applied a new in vitro selection strategy to isolate structure-switching RNA aptamers responsive to the aminoglycoside antibiotic tobramycin. Here, we report the results of mutational analysis, secondary structure modeling, and ligand-specificity studies that suggest a mechanism for tobramycin-triggered structure switching.
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Authors
Jeffrey E. Vandenengel, Daniel P. Morse,